Part:BBa_K3971007
Portion of the Neutral Site 1 of the Synechococcus elongatus UTEX 2973 genome.
This part is a portion of the Neutral Site 1 of the Synechococcus elongatus UTEX 2973 genome and can be used for homology repair-based modifications in the genome at Neutral Site 1 using the pSL2680 plasmid, which is a Cpf1 based CRISPR plasmid [1]. This was one of the homology arms to Neutral Site 1 in our composite part (BBa_K3971009) which was designed to insert a promoter upstream of a reporter (sYFP2) in order to measure the strength of the promoter, through a Cpf1 CRISPR plasmid. It was also a part of our composite part (BBa_K3971010) which was designed to test the sucrose export efficiencies of the cscB (sucrose permease) transporter under different promoters.
References
1: Cpf1 Is A Versatile Tool for CRISPR Genome Editing Across Diverse Species of Cyanobacteria. Ungerer J, Pakrasi HB. Sci Rep. 2016 Dec 21;6:39681. doi: 10.1038/srep39681. 10.1038/srep39681 PubMed 28000776
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 353
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 353
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 353
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 353
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 143
| None |